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鉄イオンおよび鉄錯体によるDNA鎖切断
https://ous.repo.nii.ac.jp/records/898
https://ous.repo.nii.ac.jp/records/89805aee802-b1f5-4b63-b482-3c037b074f0f
名前 / ファイル | ライセンス | アクション |
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KJ00000063451.pdf (362.8 kB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||||||||||||||||||
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公開日 | 1991-03-20 | |||||||||||||||||||||
タイトル | ||||||||||||||||||||||
言語 | ja | |||||||||||||||||||||
タイトル | 鉄イオンおよび鉄錯体によるDNA鎖切断 | |||||||||||||||||||||
タイトル | ||||||||||||||||||||||
言語 | en | |||||||||||||||||||||
タイトル | DNA Strand Breaks by Ferric Nitrilotriacetate | |||||||||||||||||||||
タイトル | ||||||||||||||||||||||
言語 | ja-Kana | |||||||||||||||||||||
タイトル | テツ イオン オヨビ テツ サクタイ ニヨル DNAサ セツダン | |||||||||||||||||||||
言語 | ||||||||||||||||||||||
言語 | jpn | |||||||||||||||||||||
資源タイプ | ||||||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
資源タイプ | departmental bulletin paper | |||||||||||||||||||||
ページ属性 | ||||||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||||||
内容記述 | P(論文) | |||||||||||||||||||||
著者名 |
張, 清敏
× 張, 清敏
× 池田, 正五
× 小田, 琢三
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著者所属(日) | ||||||||||||||||||||||
ja | ||||||||||||||||||||||
岡山理科大学理学部生物化学科 | ||||||||||||||||||||||
著者所属(日) | ||||||||||||||||||||||
ja | ||||||||||||||||||||||
岡山理科大学理学部生物化学科 | ||||||||||||||||||||||
著者所属(日) | ||||||||||||||||||||||
ja | ||||||||||||||||||||||
岡山理科大学理学部生物化学科 | ||||||||||||||||||||||
著者所属(英) | ||||||||||||||||||||||
en | ||||||||||||||||||||||
Department of Biochemistry, Faculty of Science, Okayama University of Science | ||||||||||||||||||||||
著者所属(英) | ||||||||||||||||||||||
en | ||||||||||||||||||||||
Department of Biochemistry, Faculty of Science, Okayama University of Science | ||||||||||||||||||||||
著者所属(英) | ||||||||||||||||||||||
en | ||||||||||||||||||||||
Department of Biochemistry, Faculty of Science, Okayama University of Science | ||||||||||||||||||||||
抄録(英) | ||||||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||||||
内容記述 | Ferric nitrilotriacetate (Fe^<3+>-NTA) is an iron complex. Repeated injections of Fe^<3+>-NTA in experimental animals induce iron overload and injuries of several organs. Fe^<3+>-NTA is also a potent carcinogen and has been shown to cause renal cell carinoma in the rat. In the present study, we investigated DNA strand breaks induced by Fe^<3+>-NTA in vitro by use of supercoiled closed circular DNA of plasmid pUC 19. The conformational change of DNA caused by a singel strand or double strand DNA break was easily detected by agarose gle electrophoresis. The DNA strand breaks were not observed with Fe^<3+>-NTA alone. However in the presence of 10mM hydrogen peroxide or 10mM dithiothreitol Fe^<3+>-NTA, at more than 10^<-4>M, caused DNA cleavage. Decrease of form I DNA and increase of form II and form III DNAs were observed by raising the Fe^<3+>-NTA concentration in the reaction. The requirement of hydrogen peroxide for the cleavage of DNA by Fe^<3+>-NTA suggested that Fe^<3+>-NTA catalyzed the production of hydroxyl radicals from hydrogen peroxide and the radicals caused the DNA strand breaks. | |||||||||||||||||||||
言語 | en | |||||||||||||||||||||
雑誌書誌ID | ||||||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||||||
収録物識別子 | AN00033244 | |||||||||||||||||||||
書誌情報 |
ja : 岡山理科大学紀要. A, 自然科学 en : Bulletin of Okayama University of Science. A, Natural Sciences 巻 26, p. 117-124, 発行日 1991-03-20 |