{"created":"2023-06-19T10:35:00.990932+00:00","id":898,"links":{},"metadata":{"_buckets":{"deposit":"c6a0348d-19cf-48fe-84a2-16c0578e3408"},"_deposit":{"created_by":14,"id":"898","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"898"},"status":"published"},"_oai":{"id":"oai:ous.repo.nii.ac.jp:00000898","sets":["296:314:324"]},"author_link":[],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-03-20","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"124","bibliographicPageStart":"117","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"岡山理科大学紀要. A, 自然科学","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of Okayama University of Science. A, Natural Sciences","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"張, 清敏","creatorNameLang":"ja"},{"creatorName":"Zhang, Qingmin","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"池田, 正五","creatorNameLang":"ja"},{"creatorName":"イケダ, ショウゴ","creatorNameLang":"ja-Kana"},{"creatorName":"Ikeda, Shogo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"小田, 琢三","creatorNameLang":"ja"},{"creatorName":"オダ, タクゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"Oda, Takuzo","creatorNameLang":"en"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Ferric nitrilotriacetate (Fe^<3+>-NTA) is an iron complex. Repeated injections of Fe^<3+>-NTA in experimental animals induce iron overload and injuries of several organs. Fe^<3+>-NTA is also a potent carcinogen and has been shown to cause renal cell carinoma in the rat. In the present study, we investigated DNA strand breaks induced by Fe^<3+>-NTA in vitro by use of supercoiled closed circular DNA of plasmid pUC 19. The conformational change of DNA caused by a singel strand or double strand DNA break was easily detected by agarose gle electrophoresis. The DNA strand breaks were not observed with Fe^<3+>-NTA alone. However in the presence of 10mM hydrogen peroxide or 10mM dithiothreitol Fe^<3+>-NTA, at more than 10^<-4>M, caused DNA cleavage. Decrease of form I DNA and increase of form II and form III DNAs were observed by raising the Fe^<3+>-NTA concentration in the reaction. The requirement of hydrogen peroxide for the cleavage of DNA by Fe^<3+>-NTA suggested that Fe^<3+>-NTA catalyzed the production of hydroxyl radicals from hydrogen peroxide and the radicals caused the DNA strand breaks.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033244","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Faculty of Science, Okayama University of Science"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Faculty of Science, Okayama University of Science"},{"subitem_text_language":"en","subitem_text_value":"Department of Biochemistry, Faculty of Science, Okayama University of Science"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"岡山理科大学理学部生物化学科"},{"subitem_text_language":"ja","subitem_text_value":"岡山理科大学理学部生物化学科"},{"subitem_text_language":"ja","subitem_text_value":"岡山理科大学理学部生物化学科"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1991-03-20"}],"displaytype":"detail","filename":"KJ00000063451.pdf","filesize":[{"value":"362.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://ous.repo.nii.ac.jp/record/898/files/KJ00000063451.pdf"},"version_id":"8933c41d-894e-456f-a207-d1384b896f3c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"鉄イオンおよび鉄錯体によるDNA鎖切断","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"鉄イオンおよび鉄錯体によるDNA鎖切断","subitem_title_language":"ja"},{"subitem_title":"DNA Strand Breaks by Ferric Nitrilotriacetate","subitem_title_language":"en"},{"subitem_title":"テツ イオン オヨビ テツ サクタイ ニヨル DNAサ セツダン","subitem_title_language":"ja-Kana"}]},"item_type_id":"1","owner":"14","path":["324"],"pubdate":{"attribute_name":"PubDate","attribute_value":"1991-03-20"},"publish_date":"1991-03-20","publish_status":"0","recid":"898","relation_version_is_last":true,"title":["鉄イオンおよび鉄錯体によるDNA鎖切断"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-09-27T06:27:53.906626+00:00"}