@article{oai:ous.repo.nii.ac.jp:00000898,
 author = {張, 清敏 and Zhang, Qingmin and 池田, 正五 and Ikeda, Shogo and 小田, 琢三 and Oda, Takuzo},
 journal = {岡山理科大学紀要. A, 自然科学, Bulletin of Okayama University of Science. A, Natural Sciences},
 month = {Mar},
 note = {P(論文), Ferric nitrilotriacetate (Fe^<3+>-NTA) is an iron complex. Repeated injections of Fe^<3+>-NTA in experimental animals induce iron overload and injuries of several organs. Fe^<3+>-NTA is also a potent carcinogen and has been shown to cause renal cell carinoma in the rat. In the present study, we investigated DNA strand breaks induced by Fe^<3+>-NTA in vitro by use of supercoiled closed circular DNA of plasmid pUC 19. The conformational change of DNA caused by a singel strand or double strand DNA break was easily detected by agarose gle electrophoresis. The DNA strand breaks were not observed with Fe^<3+>-NTA alone. However in the presence of 10mM hydrogen peroxide or 10mM dithiothreitol Fe^<3+>-NTA, at more than 10^<-4>M, caused DNA cleavage. Decrease of form I DNA and increase of form II and form III DNAs were observed by raising the Fe^<3+>-NTA concentration in the reaction. The requirement of hydrogen peroxide for the cleavage of DNA by Fe^<3+>-NTA suggested that Fe^<3+>-NTA catalyzed the production of hydroxyl radicals from hydrogen peroxide and the radicals caused the DNA strand breaks.},
 pages = {117--124},
 title = {鉄イオンおよび鉄錯体によるDNA鎖切断},
 volume = {26},
 year = {1991},
 yomi = {イケダ, ショウゴ and オダ, タクゾウ}
}