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ポリアクリルアミドゲルによるパパインの固定化
https://ous.repo.nii.ac.jp/records/637
https://ous.repo.nii.ac.jp/records/6377def0262-7970-48cb-8fcf-4aec343829c5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000063190.pdf (572.8 kB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 1982-03-05 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | ポリアクリルアミドゲルによるパパインの固定化 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Immobilization of Papain by the Use of Polyacrylamide | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | ポリアクリルアミド ゲル ニヨル パパイン ノ コテイカ | |||||||||||||||||
| 言語 | ja-Kana | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| ページ属性 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | P(論文) | |||||||||||||||||
| 著者名 |
河邊, 誠一郎
× 河邊, 誠一郎
× 河邊, 要太郎
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| 著者所属(日) | ||||||||||||||||||
| ja | ||||||||||||||||||
| 岡山理科大学基礎理学科 | ||||||||||||||||||
| 著者所属(日) | ||||||||||||||||||
| ja | ||||||||||||||||||
| 岡山理科大学応用化学科 | ||||||||||||||||||
| 著者所属(英) | ||||||||||||||||||
| en | ||||||||||||||||||
| Department of Fundamental Natural Science, Okayama University of Science | ||||||||||||||||||
| 著者所属(英) | ||||||||||||||||||
| en | ||||||||||||||||||
| Department of Applied Chemistry, Okayama University of Science | ||||||||||||||||||
| 抄録(英) | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | Papain was immobilized in crosslinked polyacrylamide gel by entrapment. The method is based on the polymerization of acrylamide in the presence of varying amount of N, N'-methylene bisacrylamide, 3-dimethyl aminopropionitrile and potassumpersulfate as crosslinking monomer, polymerization accelerator and initiator, in an aqueous medium containing the dissolved papain. It appeared that the only acrylamide as polymerization monomer decreased the activity of papain. Then, to protect papain against the denaturating agent, mercury chloride was used for protective agent. The papain activity was completely protected by this modified it's active site (sulfhydril radical : -SH) with mercury. The apparent enzyme activity of acrylamide-gel entrapped mercury papain was about 24% (15.6% after three batch reactions) as compared with native papain activty. On the other hand, the activity of gel entrapped untreated papain was only 7.3% (7.0% after three batch reactions). The enzyme characteristics of gel entrapped papain was compared with that of native papain. Temperature and pH optima were not affected by the immobilization. The remaining activity of gel entrapped mercury papain showed about 32% of the original activity after 30 batch reactions at 35℃ for 20 min. In these reactions, it was found that only 45～50% of the original activity was retained until three batch reactions. Whereas the original activity of the gel entrapped untreated papain was retained only 28% of the gel entrapped mercury papain activity. However, about 70% of the original activity was still left, after 20 batch reactions at 35℃ for 20 min. However immobilization of mercury papain resulted in a grater amount of elimination, at the end of the batch reaction, the activity of the gel entrapped mercury papain showed 1.7 times that of the gel entrapped untreated papain. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 雑誌書誌ID | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AN00033244 | |||||||||||||||||
| 書誌情報 |
ja : 岡山理科大学紀要. A, 自然科学 en : The Bulletin of the Okayama University of Science, A, Natural Science 巻 17, p. 45-56, 発行日 1982-03-05 |
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