@article{oai:ous.repo.nii.ac.jp:00000637,
 author = {河邊, 誠一郎 and Kawabe, Seiichiro and 河邊, 要太郎 and Kawabe, Yotaro},
 journal = {岡山理科大学紀要. A, 自然科学, The Bulletin of the Okayama University of Science, A, Natural  Science},
 month = {Mar},
 note = {P(論文), Papain was immobilized in crosslinked polyacrylamide gel by entrapment. The method is based on the polymerization of acrylamide in the presence of varying amount of N, N'-methylene bisacrylamide, 3-dimethyl aminopropionitrile and potassumpersulfate as crosslinking monomer, polymerization accelerator and initiator, in an aqueous medium containing the dissolved papain. It appeared that the only acrylamide as polymerization monomer decreased the activity of papain. Then, to protect papain against the denaturating agent, mercury chloride was used for protective agent. The papain activity was completely protected by this modified it's active site (sulfhydril radical : -SH) with mercury. The apparent enzyme activity of acrylamide-gel entrapped mercury papain was about 24% (15.6% after three batch reactions) as compared with native papain activty. On the other hand, the activity of gel entrapped untreated papain was only 7.3% (7.0% after three batch reactions). The enzyme characteristics of gel entrapped papain was compared with that of native papain. Temperature and pH optima were not affected by the immobilization. The remaining activity of gel entrapped mercury papain showed about 32% of the original activity after 30 batch reactions at 35℃ for 20 min. In these reactions, it was found that only 45～50% of the original activity was retained until three batch reactions. Whereas the original activity of the gel entrapped untreated papain was retained only 28% of the gel entrapped mercury papain activity. However, about 70% of the original activity was still left, after 20 batch reactions at 35℃ for 20 min. However immobilization of mercury papain resulted in a grater amount of elimination, at the end of the batch reaction, the activity of the gel entrapped mercury papain showed 1.7 times that of the gel entrapped untreated papain.},
 pages = {45--56},
 title = {ポリアクリルアミドゲルによるパパインの固定化},
 volume = {17},
 year = {1982},
 yomi = {カワベ, セイイチロウ and カワベ, ヨウタロウ}
}