@article{oai:ous.repo.nii.ac.jp:00000742,
 author = {浜田, 博喜 and Hamada, Hiroki and 山崎, 慶子 and Yamasaki, Keiko and 河邊, 誠一郎 and Kawabe, Seiichiro},
 journal = {岡山理科大学紀要. A, 自然科学, Bulletin of Okayama University of Science. A, Natural Sciences},
 month = {Mar},
 note = {P(論文), We studied callus formation and plant regeneration from Nicotiana tabacum "Mild Cure". Somatic embryogenesis and subsequent formation of plantlets were obtained from callus cultures derived from leaves of Nicotiana tabacum "Mild Cure". The callus was induced from leaf explants and grown on Murashige and Skoog's medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) 2 ppm and 3% sucrose. Reduction of 2,4-D concentration during subsequent subcultures resulted in formation of embryoids. On the other hand shoots and plantlets were generated when the auxin-free medium formed the embryoid was transferred to medium supplemented with 3% sucrose.},
 pages = {7--11},
 title = {Callus Formation and Plant Regeneration from Nicotiana tabacum "Mild Cure"},
 volume = {22},
 year = {1987},
 yomi = {ハマダ, ヒロキ and ヤマサキ, ケイコ and カワベ, セイイチロウ}
}