{"created":"2023-06-19T10:34:41.523006+00:00","id":546,"links":{},"metadata":{"_buckets":{"deposit":"e4833362-081d-42f6-a636-adc432bb767f"},"_deposit":{"created_by":14,"id":"546","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"546"},"status":"published"},"_oai":{"id":"oai:ous.repo.nii.ac.jp:00000546","sets":["296:297:310"]},"author_link":["17820"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1978-03-05","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"130","bibliographicPageStart":"119","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"岡山理科大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"The Bulletin of the Okayama University of Science,","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"河邊 誠一郎","creatorNameLang":"ja"},{"creatorName":"カワベ, セイイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawabe, Seiichiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Active-ionizing group and reaction mechanism of papain were investigated by using the following four substrates ; Casein, N-α-Benzoyl-DL-arginin-p-nitroanilide hydrochloride (BAPA), N-α-Benzoyl-L-arginin ethyl ester hydrochloride (BAEE), and N-Benzoyl-L-tyrosine ethyl ester (BTEE). The specificity of papain for various substrates was investigated on the basis of the reaction initial velocity and the kinetics constants were determined by reaction pH values. And then pK values were determined by using Dixon plot. From these results, in case BAPA and Casein were used, active-ionizing groups and reaction mechanism of papain were estimated as follows : A). Active-ionizing group of papain ; BAPA : Sulphydryl, Carboxyl. Casein : Sulphydryl, Imidazole. B). Reaction mechanism of papain ; refer to Fig.6.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00033233","subitem_source_identifier_type":"NCID"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"岡山理科大学基礎理学科"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1978-03-05"}],"displaytype":"detail","filename":"KJ00000062962.pdf","filesize":[{"value":"568.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://ous.repo.nii.ac.jp/record/546/files/KJ00000062962.pdf"},"version_id":"ade9b3c7-e8d6-4ce4-a150-27b3cc4392f4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"パパインの反応機構について : その反応動力学定数による推定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"パパインの反応機構について : その反応動力学定数による推定","subitem_title_language":"ja"},{"subitem_title":"Reaction Mechanism of Papain : Estimation of its Mechanism from Kinetics Constant","subitem_title_language":"en"},{"subitem_title":"パパイン ノ ハンノウ キコウ ニツイテ ソノ ハンノウ ドウリョクガク テイスウ ニヨル スイテイ","subitem_title_language":"ja-Kana"}]},"item_type_id":"1","owner":"14","path":["310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"1978-03-05"},"publish_date":"1978-03-05","publish_status":"0","recid":"546","relation_version_is_last":true,"title":["パパインの反応機構について : その反応動力学定数による推定"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-10-03T07:58:31.439842+00:00"}